Mpho Koaho was born in Toronto, Ontario, Canada to South African parents. He began his acting career when Maya Angelou cast him as the lead in her 1998 Miramax film Down in the Delta. D.J. Caruso next cast Koaho in The Salton Sea, opposite Val Kilmer. Since then, Koaho has gone on to co-star in Four Brothers, Get Rich or Die Tryin’ , Blindness, Saw III and Saw VI. Koaho also portrayed Anthony, a series regular on the TV series Falling Skies produced by Steven Spielberg, which began airing in June 2011.
In 2009, Koaho was nominated to two Gemini Awards – one for his supporting role on the Canadian TV series Soul and another for his critically acclaimed guest appearance on the TV series Flashpoint. He ended up winning the former.